PT  - JOURNAL ARTICLE
AU  - Curti Lucia
AU  - Pereyra-Bonnet Federico
AU  - Gimenez Carla Alejandra
TI  - An ultrasensitive, rapid, and portable coronavirus SARS-CoV-2 sequence detection method based on CRISPR-Cas12
AID  - 10.1101/2020.02.29.971127
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.02.29.971127
4099  - http://biorxiv.org/content/early/2020/03/02/2020.02.29.971127.short
4100  - http://biorxiv.org/content/early/2020/03/02/2020.02.29.971127.full
AB  - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has received global attention due to the recent outbreak in China. In this work, we report a CRISPR-Cas12 based diagnostic tool to detect synthetic SARS-CoV-2 RNA sequences in a proof-of-principle evaluation. The test proved to be sensitive, rapid, and potentially portable. These key traits of the CRISPR method are critical for virus detection in regions that lack resources to use the currently available methods.