RT Journal Article SR Electronic T1 Mapping the immunogenic landscape of near-native HIV-1 envelope trimers in non-human primates JF bioRxiv FD Cold Spring Harbor Laboratory SP 2020.02.05.936096 DO 10.1101/2020.02.05.936096 A1 Christopher A. Cottrell A1 Jelle van Schooten A1 Charles A. Bowman A1 Meng Yuan A1 David Oyen A1 Mia Shin A1 Robert Morpurgo A1 Patricia van der Woude A1 Marielle van Breemen A1 Jonathan L. Torres A1 Raj Patel A1 Justin Gross A1 Leigh M. Sewall A1 Jeffrey Copps A1 Gabriel Ozorowski A1 Bartek Nogal A1 Devin Sok A1 Eva G. Rakasz A1 Celia Labranche A1 Vladimir Vigdorovich A1 Scott Christley A1 Diane G. Carnathan A1 D. Noah Sather A1 David Montefiori A1 Guido Silvestri A1 Dennis R. Burton A1 John P. Moore A1 Ian A. Wilson A1 Rogier W. Sanders A1 Andrew B. Ward A1 Marit J. van Gils YR 2020 UL http://biorxiv.org/content/early/2020/03/02/2020.02.05.936096.abstract AB The induction of broad and potent immunity by vaccines is the key focus of research efforts aimed at protecting against HIV-1 infection. Soluble native-like HIV-1 envelope glycoproteins have shown promise as vaccine candidates as they can induce potent autologous neutralizing responses in rabbits and non-human primates. In this study, monoclonal antibodies were isolated and characterized from rhesus macaques immunized with the BG505 SOSIP.664 trimer to better understand vaccine-induced antibody responses. Our studies reveal a diverse landscape of antibodies recognizing immunodominant strain-specific epitopes and non-neutralizing neo-epitopes. Additionally, we isolated a subset of mAbs against an epitope cluster at the gp120-gp41 interface that recognize the highly conserved fusion peptide and the glycan at position 88 and have characteristics akin to several human-derived broadly neutralizing antibodies.