PT  - JOURNAL ARTICLE
AU  - Aron N. Horvath
AU  - Andreas A. Ziegler
AU  - Stephan Gerhard
AU  - Claude N. Holenstein
AU  - Benjamin Beyeler
AU  - Jess G. Snedeker
AU  - Unai Silvan
TI  - Time-controlled Multichannel Dynamic Traction Imaging of Biaxially Stretched Adherent Cells
AID  - 10.1101/2020.03.02.972919
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.03.02.972919
4099  - http://biorxiv.org/content/early/2020/03/03/2020.03.02.972919.short
4100  - http://biorxiv.org/content/early/2020/03/03/2020.03.02.972919.full
AB  - Here, a dynamic traction force microscopy method is described which enables sub-second temporal resolution imaging of transient subcellular events secondary to extrinsic stretch of adherent single cells. The system employs a novel tracking approach with minimal computational overhead to compensate substrate-based stretch-induced motion/drift of stretched single cells in real time, allowing capture of biophysical phenomena on multiple channels by fluorescent multichannel imaging on a single camera, thus avoiding the need for beam splitting with associated loss of light. The potential impact of the technique is demonstrated by characterizing transient subcellular forces and corresponding nuclear deformations in equibiaxial stretching experiments, uncovering a high frequency strain-rate dependent response in the transfer of substrate strains to the nucleus.