RT Journal Article
SR Electronic
T1 Time-controlled Multichannel Dynamic Traction Imaging of Biaxially Stretched Adherent Cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.03.02.972919
DO 10.1101/2020.03.02.972919
A1 Aron N. Horvath
A1 Andreas A. Ziegler
A1 Stephan Gerhard
A1 Claude N. Holenstein
A1 Benjamin Beyeler
A1 Jess G. Snedeker
A1 Unai Silvan
YR 2020
UL http://biorxiv.org/content/early/2020/03/03/2020.03.02.972919.abstract
AB Here, a dynamic traction force microscopy method is described which enables sub-second temporal resolution imaging of transient subcellular events secondary to extrinsic stretch of adherent single cells. The system employs a novel tracking approach with minimal computational overhead to compensate substrate-based stretch-induced motion/drift of stretched single cells in real time, allowing capture of biophysical phenomena on multiple channels by fluorescent multichannel imaging on a single camera, thus avoiding the need for beam splitting with associated loss of light. The potential impact of the technique is demonstrated by characterizing transient subcellular forces and corresponding nuclear deformations in equibiaxial stretching experiments, uncovering a high frequency strain-rate dependent response in the transfer of substrate strains to the nucleus.