TY - JOUR T1 - Defining totipotency using criteria of increasing stringency JF - bioRxiv DO - 10.1101/2020.03.02.972893 SP - 2020.03.02.972893 AU - Eszter Posfai AU - John Paul Schell AU - Adrian Janiszewski AU - Isidora Rovic AU - Alexander Murray AU - Brian Bradshaw AU - Tine Pardon AU - Mouna El Bakkali AU - Irene Talon AU - Natalie De Geest AU - Pankaj Kumar AU - San Kit To AU - Sophie Petropoulos AU - Andrea Jurisicova AU - Vincent Pasque AU - Fredrik Lanner AU - Janet Rossant Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/03/03/2020.03.02.972893.abstract N2 - Totipotency is the ability of a single cell to give rise to all the differentiated cells that build the conceptus, yet how to capture this property in vitro remains incompletely understood. Defining totipotency relies upon a variety of assays of variable stringency. Here we describe criteria to define totipotency. We illustrate how distinct criteria of increasing stringency can be used to judge totipotency by evaluating candidate totipotent cell types in the mouse, including early blastomeres and expanded or extended pluripotent stem cells. Our data challenge the notion that expanded or extended pluripotent states harbor increased totipotent potential relative to conventional embryonic stem cells under in vivo conditions. ER -