RT Journal Article
SR Electronic
T1 Assessing sub-cellular resolution in spatial proteomics experiments
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 377630
DO 10.1101/377630
A1 Laurent Gatto
A1 Lisa M. Breckels
A1 Kathryn S. Lilley
YR 2018
UL http://biorxiv.org/content/early/2018/08/10/377630.abstract
AB The sub-cellular localisation of a protein is vital in defining its function, and a protein’s mis-localisation is known to lead to adverse effect. As a result, numerous experimental techniques and datasets have been published, with the aim of deciphering the localisation of proteins at various scales and resolutions, including high profile mass spectrometry-based efforts. Here, we present a meta-analysis assessing and comparing the sub-cellular resolution of 29 such mass spectrometry-based spatial proteomics experiments using a newly developed tool termed QSep. Our goal is to provide a simple quantitative report of how well spatial proteomics resolve the sub-cellular niches they describe to inform and guide developers and users of such methods.