RT Journal Article
SR Electronic
T1 Extreme phenotypes define epigenetic and metabolic signatures in cardiometabolic syndrome
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.03.06.961805
DO 10.1101/2020.03.06.961805
A1 Denis Seyres
A1 Alessandra Cabassi
A1 John Lambourne
A1 Frances Burden
A1 Samantha Farrow
A1 Harriet McKinney
A1 Joana Batista
A1 Carly Kempster
A1 Maik Pietzner
A1 Oliver Slingsby
A1 Thong Huy Cao
A1 Paulene Quinn
A1 Luca Stefanucci
A1 Matthew C Sims
A1 Karola Rehnstrom
A1 Claire Adams
A1 Amy Frary
A1 Bekir Erguener
A1 Roman Kreuzhuber
A1 Gabriele Mocciaro
A1 Michael Allison
A1 Simona D’Amore
A1 Albert Koulman
A1 Luigi Grassi
A1 Julian L Griffin
A1 Leong Loke Ng
A1 Adrian Park
A1 David B Savage
A1 Claudia Langenberg
A1 Christoph Bock
A1 Kate Downes
A1 Michele Vacca
A1 Paul DW Kirk
A1 Mattia Frontini
YR 2020
UL http://biorxiv.org/content/early/2020/03/06/2020.03.06.961805.abstract
AB Providing a molecular characterisation of cardiometabolic syndrome (CMS) could improve our understanding of its pathogenesis and pathophysiology, and provide a step toward the development of better treatments. To this end, we performed a deep phenotyping analysis of 185 blood donors, 10 obese, and 10 lipodystrophy patients. We analysed transcriptomes and epigenomes of monocytes, neutrophils, macrophages and platelets. Additionally, plasma metabolites including lipids and biochemistry measurements were quantified.Multi-omics integration of this data allowed us to identify combinations of features related to patient status and to order the donor population according to their molecular similarity to patients. We also performed differential analyses on epigenomic, transcriptomic and plasma proteomic data collected from obese individuals before and six months after bariatric surgery. These analyses revealed a pattern of abnormal activation of immune cells in obese individuals and lipodystrophy patients, which was partially reverted six months after bariatric surgery.