RT Journal Article
SR Electronic
T1 Analysis and comparison of genome editing using CRISPResso2
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 392217
DO 10.1101/392217
A1 Kendell Clement
A1 Holly Rees
A1 Matthew C. Canver
A1 Jason M. Gehrke
A1 Rick Farouni
A1 Jonathan Y Hsu
A1 Mitchel Cole
A1 David R. Liu
A1 J. Keith Joung
A1 Daniel E. Bauer
A1 Luca Pinello
YR 2018
UL http://biorxiv.org/content/early/2018/08/15/392217.abstract
AB Genome editing technologies are rapidly evolving, and analysis of deep sequencing data from target or off-target regions is necessary for measuring editing efficiency and evaluating safety. However, no software exists to analyze base editors, perform allele-specific quantification or that incorporates biologically-informed and scalable alignment approaches. Here, we present CRISPResso2 to fill this gap and illustrate its functionality by experimentally measuring and analyzing the editing properties of six genome editing agents.