RT Journal Article
SR Electronic
T1 Narrowly confined and glomerulus-specific onset latencies of odor-evoked calcium transients in the periglomerular cells of the mouse main olfactory bulb
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 392274
DO 10.1101/392274
A1 Ryota Homma
A1 Xiaohua Lv
A1 Tokiharu Sato
A1 Fumiaki Imamura
A1 Shaoqun Zeng
A1 Shin Nagayama
YR 2018
UL http://biorxiv.org/content/early/2018/08/15/392274.abstract
AB Odor information is transmitted from olfactory sensory neurons to principal neurons at the glomeruli of the olfactory bulb. The intraglomerular neuronal circuit also includes hundreds of GABAergic interneurons referred to as periglomerular (PG) cells. Stimulus selectivity is well correlated among PG cells that are associated with the same glomerulus, consistent with their highly homogeneous sensory inputs. However, much less is known about the temporal aspects of their activity, including the temporal coordination of their odor-evoked responses. As many PG cells within a glomerular module respond to the same stimulus, the extent to which their activity is temporally aligned will affect the temporal profile of their population inhibitory inputs. Using random-access high-speed two-photon microscopy, we recorded the odor-evoked calcium transients of mouse PG cells and compared the onset latency and rise time among neurons putatively associated with the same and different glomeruli. Whereas the overall onset latencies of odor-evoked transients were distributed across a ~150 ms time window, those from cells putatively associated with the same glomerulus were confined to a much narrower window of several tens of milliseconds. This result suggests that onset latency primarily depends on the associated glomerulus. We also observed glomerular specificity in the rise time. The glomerulus-specific temporal pattern of odor-evoked activity implies that the temporal patterns of inhibitory inputs are unique to individual glomerulus–odor pairs, which may contribute to efficient shaping of the temporal pattern of activity in the principal neurons.We thank Wei Chen for his contribution to the setup of the acousto-optic deflector two-photon microscope. S.N. is supported by NIH/NIDCD Grant R01DC013802. F.I. is supported by NIH/NIDCD Grant R01DC016307. S.Z. and X.L. are supported by NSFC Grant 81327802.