RT Journal Article
SR Electronic
T1 Filamentous Bacteriophage Delay Healing of Pseudomonas-Infected Wounds
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.03.10.985663
DO 10.1101/2020.03.10.985663
A1 Michelle S. Bach
A1 Christiaan R. de Vries
A1 Johanna M. Sweere
A1 Medeea Popescu
A1 Jonas D. Van Belleghem
A1 Gernot Kaber
A1 Elizabeth B. Burgener
A1 Dan Liu
A1 Quynh-Lam Tran
A1 Tejas Dharmaraj
A1 Maria Birukova
A1 Vivekananda Sunkari
A1 Swathi Balaji
A1 Sundeep Keswani
A1 Niaz Banaei
A1 Dolly K. Khona
A1 Laurence Nedelec
A1 Chandan K. Sen
A1 Venita Chandra
A1 Patrick R. Secor
A1 Gina A. Suh
A1 Paul L. Bollyky
YR 2020
UL http://biorxiv.org/content/early/2020/03/11/2020.03.10.985663.abstract
AB We have identified a novel role for filamentous bacteriophage in the delayed healing associated with chronic Pseudomonas aeruginosa (Pa) wound infections. In a mouse model of chronic Pa-infected wounds, Pf, a filamentous phage produced by Pa, impaired keratinocyte migration, prevented wound re-epithelialization, and delayed healing in both the absence and presence of live bacteria. Mechanistically, the immune response to Pf phage produces soluble factors that impair keratinocyte migration and delay wound re-epithelialization. In a prospective cohort study of 113 human patients, Pa was detected in 36 patients and 25 of these (69%) were positive for Pf phage. Pf(+) wounds were significantly older and more likely to increase in size over time than Pf(-) wounds. Together, these data implicate Pf in the delayed wound healing associated with Pa infection. We propose that Pf phage may have potential as a biomarker and therapeutic target for delayed wound healing.