RT Journal Article
SR Electronic
T1 In vivo detection of optically-evoked opioid peptide release
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 393603
DO 10.1101/393603
A1 Ream Al-Hasanil
A1 Jenny-Marie T. Wong
A1 Omar S. Mabrouk
A1 Jordan G. McCall
A1 Gavin P. Schmitz
A1 Kirsten A. Porter-Stransky
A1 Brandon J. Aragona
A1 Robert T. Kennedy
A1 Michael R. Bruchas
YR 2018
UL http://biorxiv.org/content/early/2018/08/16/393603.abstract
AB Though the last decade has seen accelerated advances in techniques and technologies to perturb neuronal circuitry in the brain, we are still poorly equipped to adequately dissect endogenous peptide release in vivo. To this end we developed a system that combines in vivo optogenetics with microdialysis and a highly sensitive mass spectrometry-based assay to measure opioid peptide release in freely moving rodents.