@article {Barajas2020.03.11.988014, author = {Hugo R. Barajas and Shamayim Mart{\'\i}nez-S{\'a}nchez and Miguel F. Romero and Crist{\'o}bal Hern{\'a}ndez-{\'A}lvarez and Luis Serv{\'\i}n-Gonz{\'a}lez and Mariana Peimbert and Roc{\'\i}o Cruz-Ortega and Felipe Garc{\'\i}a-Oliva and Luis D. Alcaraz}, title = {Testing the two-step model of plant root microbiome acquisition under multiple plant species and soil sources}, elocation-id = {2020.03.11.988014}, year = {2020}, doi = {10.1101/2020.03.11.988014}, publisher = {Cold Spring Harbor Laboratory}, abstract = {The two-step model for plant root microbiomes considers soil as the primary microbial source. Active selection of the plant{\textquoteright}s bacterial inhabitants results in a biodiversity decrease towards roots. We collected in situ ruderal plant roots and their soils and used these soils as the main microbial input for single genotype tomatoes grown in a greenhouse. We massively sequenced the 16S rRNA and shotgun metagenomes of the soils, in situ plants, and tomato roots. Tomato roots did follow the two-step model, while ruderal plants did not. Ruderal plants and their soils are closer than tomato and its soil, based on protein comparisons. We calculated a metagenomic tomato root core of 51 bacterial genera and 2,762 proteins, which could be the basis for microbiome-oriented plant breeding programs. The tomato and ruderal metagenomic differences are probably due to plant domestication trade-offs, impacting plant-microbe interactions.}, URL = {https://www.biorxiv.org/content/early/2020/03/12/2020.03.11.988014}, eprint = {https://www.biorxiv.org/content/early/2020/03/12/2020.03.11.988014.full.pdf}, journal = {bioRxiv} }