PT - JOURNAL ARTICLE AU - Harris, Lachlan AU - Rigo, Piero AU - Stiehl, Thomas AU - Gaber, Zachary AU - Austin, Sophie H. L. AU - del Mar Masdeu, Maria AU - Edwards, Amelia AU - Urbán, Noelia AU - Marciniak-Czochra, Anna AU - Guillemot, François TI - Progressive changes in hippocampal stem cell properties ensure lifelong neurogenesis AID - 10.1101/2020.03.12.987107 DP - 2020 Jan 01 TA - bioRxiv PG - 2020.03.12.987107 4099 - http://biorxiv.org/content/early/2020/03/12/2020.03.12.987107.short 4100 - http://biorxiv.org/content/early/2020/03/12/2020.03.12.987107.full AB - Neural stem cell numbers fall rapidly in the hippocampus of juvenile mice but stabilise during adulthood, ensuring lifelong hippocampal neurogenesis. We show that this reduction in stem cell depletion rate is the result of multiple coordinated changes in stem cell behaviour. In particular, while active neural stem cells divide only once or twice before differentiating rapidly in juveniles, they increasingly return to a resting state of shallow quiescence and progress through additional self-renewing divisions in adulthood. Single-cell transcriptomic, mathematical modelling and label-retention analyses indicate that resting cells have a higher activation rate and greater contribution to neurogenesis than dormant cells, which have not left quiescence. These progressive changes in stem cell behaviour result from reduced expression of the pro-activation protein ASCL1 due to increased post-translational degradation. These mechanisms help reconcile current contradictory models of hippocampal NSC dynamics and may contribute to the different rates of decline of hippocampal neurogenesis in mammalian species including humans.