PT  - JOURNAL ARTICLE
AU  - Murat Sunbul
AU  - Jens Lackner
AU  - Annabell Martin
AU  - Daniel Englert
AU  - Benjamin Hacene
AU  - Karin Nienhaus
AU  - G. Ulrich Nienhaus
AU  - Andres Jäschke
TI  - RhoBAST - a rhodamine-binding aptamer for super-resolution RNA imaging
AID  - 10.1101/2020.03.12.988782
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.03.12.988782
4099  - http://biorxiv.org/content/early/2020/03/13/2020.03.12.988782.short
4100  - http://biorxiv.org/content/early/2020/03/13/2020.03.12.988782.full
AB  - RhoBAST is a novel fluorescence light-up RNA aptamer (FLAP) that transiently binds a fluorogenic rhodamine dye. Fast dye association and dissociation result in intermittent fluorescence emission, facilitating single-molecule localization microscopy (SMLM) with an image resolution not limited by photobleaching. We demonstrate RhoBAST's excellent properties as a RNA marker by resolving subcellular and subnuclear structures of RNA in live and fixed cells by SMLM and structured illumination microscopy (SIM).