PT  - JOURNAL ARTICLE
AU  - Wong, Sarah Anne
AU  - Hu, Diane
AU  - Shao, Tiffany
AU  - Niemi, Erene
AU  - Barruet, Emilie
AU  - Morales, Blanca M
AU  - Boozarpour, Omid
AU  - Miclau, Theodore
AU  - Hsiao, Edward C
AU  - Nakamura, Mary
AU  - Bahney, Chelsea S
AU  - Marcucio, Ralph S
TI  - β-catenin Signaling Regulates Cell Fate Decisions at the Transition Zone of the Chondro-Osseous Junction During Fracture Healing
AID  - 10.1101/2020.03.11.986141
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.03.11.986141
4099  - http://biorxiv.org/content/early/2020/03/14/2020.03.11.986141.short
4100  - http://biorxiv.org/content/early/2020/03/14/2020.03.11.986141.full
AB  - Chondrocytes within the fracture callus transform into osteoblasts during bone regeneration, but the molecular mechanisms regulating this process are unknown. Wnt ligands are expressed within the fracture callus, and hypertrophic chondrocytes undergoing transformation to osteoblasts exhibit nuclear localization of β-catenin, indicating active Wnt signaling in these cells. Here, we show that conditional knock out (cKO) of β-catenin in chondrocytes inhibits the transformation of chondrocytes to osteoblasts, while stabilization of β-catenin in chondrocytes accelerates this process. After cKO, chondrocyte-derived cells were located in the bone marrow cavity and upon re-fracture formed cartilage. Lineage tracing in wild type mice revealed that in addition to osteoblasts, chondrocytes give rise to stem cells that contribute to repair of subsequent fractures. These data indicate that Wnt signaling directs cell fate choices of chondrocytes during fracture healing by stimulating transformation of chondrocytes to osteoblasts, and provide a framework for developing Wnt-therapies to stimulate repair.