RT Journal Article
SR Electronic
T1 β-catenin Signaling Regulates Cell Fate Decisions at the Transition Zone of the Chondro-Osseous Junction During Fracture Healing
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.03.11.986141
DO 10.1101/2020.03.11.986141
A1 Wong, Sarah Anne
A1 Hu, Diane
A1 Shao, Tiffany
A1 Niemi, Erene
A1 Barruet, Emilie
A1 Morales, Blanca M
A1 Boozarpour, Omid
A1 Miclau, Theodore
A1 Hsiao, Edward C
A1 Nakamura, Mary
A1 Bahney, Chelsea S
A1 Marcucio, Ralph S
YR 2020
UL http://biorxiv.org/content/early/2020/03/14/2020.03.11.986141.abstract
AB Chondrocytes within the fracture callus transform into osteoblasts during bone regeneration, but the molecular mechanisms regulating this process are unknown. Wnt ligands are expressed within the fracture callus, and hypertrophic chondrocytes undergoing transformation to osteoblasts exhibit nuclear localization of β-catenin, indicating active Wnt signaling in these cells. Here, we show that conditional knock out (cKO) of β-catenin in chondrocytes inhibits the transformation of chondrocytes to osteoblasts, while stabilization of β-catenin in chondrocytes accelerates this process. After cKO, chondrocyte-derived cells were located in the bone marrow cavity and upon re-fracture formed cartilage. Lineage tracing in wild type mice revealed that in addition to osteoblasts, chondrocytes give rise to stem cells that contribute to repair of subsequent fractures. These data indicate that Wnt signaling directs cell fate choices of chondrocytes during fracture healing by stimulating transformation of chondrocytes to osteoblasts, and provide a framework for developing Wnt-therapies to stimulate repair.