RT Journal Article
SR Electronic
T1 Reinfection could not occur in SARS-CoV-2 infected rhesus macaques
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.03.13.990226
DO 10.1101/2020.03.13.990226
A1 Bao, Linlin
A1 Deng, Wei
A1 Gao, Hong
A1 Xiao, Chong
A1 Liu, Jiayi
A1 Xue, Jing
A1 Lv, Qi
A1 Liu, Jiangning
A1 Yu, Pin
A1 Xu, Yanfeng
A1 Qi, Feifei
A1 Qu, Yajin
A1 Li, Fengdi
A1 Xiang, Zhiguang
A1 Yu, Haisheng
A1 Gong, Shuran
A1 Liu, Mingya
A1 Wang, Guanpeng
A1 Wang, Shunyi
A1 Song, Zhiqi
A1 Zhao, Wenjie
A1 Han, Yunlin
A1 Zhao, Linna
A1 Liu, Xing
A1 Wei, Qiang
A1 Qin, Chuan
YR 2020
UL http://biorxiv.org/content/early/2020/03/14/2020.03.13.990226.abstract
AB An outbreak of the Corona Virus Disease 2019 (COVID-19), caused by the severe acute respiratory syndrome CoV-2 (SARS-CoV-2), began in Wuhan and spread globally. Recently, it has been reported that discharged patients in China and elsewhere were testing positive after recovering. However, it remains unclear whether the convalescing patients have a risk of “relapse” or “reinfection”. The longitudinal tracking of re-exposure after the disappeared symptoms of the SARS-CoV-2-infected monkeys was performed in this study. We found that weight loss in some monkeys, viral replication mainly in nose, pharynx, lung and gut, as well as moderate interstitial pneumonia at 7 days post-infection (dpi) were clearly observed in rhesus monkeys after the primary infection. After the symptoms were alleviated and the specific antibody tested positively, the half of infected monkeys were rechallenged with the same dose of SARS-CoV-2 strain. Notably, neither viral loads in nasopharyngeal and anal swabs along timeline nor viral replication in all primary tissue compartments at 5 days post-reinfection (dpr) was found in re-exposed monkeys. Combined with the follow-up virologic, radiological and pathological findings, the monkeys with re-exposure showed no recurrence of COVID-19, similarly to the infected monkey without rechallenge. Taken together, our results indicated that the primary SARS-CoV-2 infection could protect from subsequent exposures, which have the reference of prognosis of the disease and vital implications for vaccine design.