RT Journal Article
SR Electronic
T1 Adaptive FDR thresholding of Fourier shell correlation for resolution estimation of cryo-EM maps
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.03.13.990705
DO 10.1101/2020.03.13.990705
A1 Beckers, Maximilian
A1 Sachse, Carsten
YR 2020
UL http://biorxiv.org/content/early/2020/03/14/2020.03.13.990705.abstract
AB Fourier shell correlation (FSC) has become a standard quantity for resolution estimation in electron cryo-microscopy. However, the resolution determination step is still subjective and not fully automated as it involves a series of map interventions before FSC computation and includes the selection of a common threshold. Here, we apply the statistical methods of permutation sampling and false discovery rate (FDR) control to the resolution-dependent correlation measure. The approach allows fully automated and mask-free resolution determination based on adaptive thresholding of FSC curves. We demonstrate the applicability for global, local and directional resolution estimation and show that the developed criterion termed FDR-FSC gives realistic resolution estimates based on a statistical significance criterion while eliminating the need of any map manipulations. The algorithms are implemented in a user-friendly GUI based software tool termed SPoC (https://github.com/MaximilianBeckers/SPOC).