PT  - JOURNAL ARTICLE
AU  - Martin Schorb
AU  - Isabella Haberbosch
AU  - Wim JH Hagen
AU  - Yannick Schwab
AU  - David N Mastronarde
TI  - Software tools for automated transmission electron dmicroscopy
AID  - 10.1101/389502
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 389502
4099  - http://biorxiv.org/content/early/2018/08/20/389502.short
4100  - http://biorxiv.org/content/early/2018/08/20/389502.full
AB  - In the recent years, electron microscopy in the life sciences has witnessed increasing demand for high-throughput data collection in both structural and cellular biology. We present a combination of software tools that enable automated acquisition guided by image analysis for a wide variety of Transmission Electron Microscopy applications. Using these tools, we demonstrate dose-reduction in single particle cryo-EM experiments, fully automated acquisition of every single cell in a plastic section and automated targeting of features on serial sections for 3D volume imaging even across multiple grids.