RT Journal Article
SR Electronic
T1 A pancreas specific <em>Ptf1a</em>-driven Cre mouse line causes paternally transmitted germline recombination
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.03.13.989178
DO 10.1101/2020.03.13.989178
A1 Derya Kabacaoglu
A1 Marina Lesina
A1 Hana Algül
YR 2020
UL http://biorxiv.org/content/early/2020/03/18/2020.03.13.989178.abstract
AB Cre-loxP recombination system is a commonly used tool to achieve site-specific genetic manipulation in genome. For multiple Cre driver mouse lines, parental transmissions of recombined flox alleles are reported. Ptf1a-driven Cre lines are widely used to achieve genetic manipulation in a pancreas specific manner. Herein, we report germline recombination in breedings when Cre allele is retained paternally in Ptf1atm1(cre)Hnak. The germline recombination frequency changed depending on the target allele. Therefore, unless the reporter allele is on the target gene, the reporter activity is to be validated. Overall, we highlight that all Ptf1a-driven Cre mouse lines should be genotyped for possible germline recombination and we advise the maternal transmission of the Cre to progeny.