@article {Valon2020.03.17.994921, author = {L{\'e}o Valon and Florence Levillayer and An{\dj}ela Davidovi{\'c} and Mathilde Chouly and Fabiana Cerqueira-Campos and Romain Levayer}, title = {Robustness of epithelial sealing is an emerging property of local ERK feedbacks driven by cell elimination}, elocation-id = {2020.03.17.994921}, year = {2020}, doi = {10.1101/2020.03.17.994921}, publisher = {Cold Spring Harbor Laboratory}, abstract = {While the pathways regulating apoptosis and cell extrusion are rather well described1,2, what regulates the precise spatio-temporal distribution of cell elimination in tissues remains largely unknown. This is particularly relevant for epithelia with high rates of cell elimination, a widespread situation during embryogenesis3-6 and homeostasis7, where concomitant death of neigbours could impair the maintenance of epithelial sealing. However, the extent to which epithelial tissues can cope with concomitant cell death, and whether any mechanism regulates such occurrence have never been explored so far. Here, using the Drosophila pupal notum (a single layer epithelium) and a new optogenetic tool to trigger caspase activation and cell extrusion, we first show that concomitant death of clusters of at least three cells is sufficient to transiently impair epithelial sealing. Such clustered extrusion was almost never observed in vivo, suggesting the existence of a mechanism preventing concomitant elimination of neighbours. Statistical analysis of cell death distribution in the notum highlighted a transient and local protective phase occurring near every dying cell. This protection is driven by a transient activation of ERK in the direct neighbours of extruding cells which reverts caspase activation and prevents elimination of cells in clusters. Altogether, this study demonstrates that the distribution of cell elimination in epithelia is an emerging property of transient and local feedbacks through ERK activation which is required to maintain epithelial sealing in conditions of high rate of cell elimination.}, URL = {https://www.biorxiv.org/content/early/2020/03/18/2020.03.17.994921}, eprint = {https://www.biorxiv.org/content/early/2020/03/18/2020.03.17.994921.full.pdf}, journal = {bioRxiv} }