RT Journal Article SR Electronic T1 Cell-type specific mechanical response and myosin dynamics during retinal lens development in Drosophila JF bioRxiv FD Cold Spring Harbor Laboratory SP 558593 DO 10.1101/558593 A1 Laura Blackie A1 Rhian F. Walther A1 Michael F. Staddon A1 Shiladitya Banerjee A1 Franck Pichaud YR 2020 UL http://biorxiv.org/content/early/2020/03/19/558593.abstract AB During organogenesis, different cell types need to work together to induce functional multicellular structures. To study this process, we made use of the genetically tractable fly retina, with a focus on the mechanisms that coordinate morphogenesis between the different epithelial cell types that make up the optical lens. Our work shows that these epithelial cells present contractile apical-medial MyosinII meshworks, which control the apical area and junctional geometry of these cells during lens development. Our study also suggests that MyosinII meshworks drive cell shape changes in response to external forces, and thus they mediate part of the biomechanical coupling that takes place between these cells. Importantly, our work, including mathematical modelling of forces and material stiffness during lens development, raises the possibility that increased cell stiffness acts as a mechanism for limiting this mechanical coupling. We propose this might be required in complex tissues, where different cell types undergo concurrent morphogenesis and where averaging out of forces across cells could compromise individual cell apical geometry and thereby organ function.