PT  - JOURNAL ARTICLE
AU  - Alsema, Astrid M.
AU  - Jiang, Qiong
AU  - Kracht, Laura
AU  - Gerrits, Emma
AU  - Dubbelaar, Marissa L.
AU  - Miedema, Anneke
AU  - Brouwer, Nieske
AU  - Woodbury, Maya
AU  - Wachter, Astrid
AU  - Xi, Hualin S.
AU  - Möller, Thomas
AU  - Biber, Knut P.
AU  - Kooistra, Susanne M.
AU  - Boddeke, Erik W.G.M
AU  - Eggen, Bart J.L.
TI  - Profiling microglia from AD donors and non-demented elderly in acute human post-mortem cortical tissue
AID  - 10.1101/2020.03.18.995332
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.03.18.995332
4099  - http://biorxiv.org/content/early/2020/03/19/2020.03.18.995332.short
4100  - http://biorxiv.org/content/early/2020/03/19/2020.03.18.995332.full
AB  - Microglia are the tissue-resident macrophages of the central nervous system (CNS). Recent studies based on bulk and single-cell RNA sequencing in mice indicate high relevance of microglia with respect to risk genes and neuro-inflammation in Alzheimer’s disease. Here, we investigated microglia transcriptomes at bulk and single cell level in non-demented elderly and AD donors using acute human post-mortem cortical brain samples. We identified 9 human microglial subpopulations with heterogeneity in gene expression. Notably, gene expression profiles and subcluster composition of microglia did not differ between AD donors and non-demented elderly in bulk RNA sequencing nor in single-cell sequencing.