RT Journal Article
SR Electronic
T1 Profiling microglia from AD donors and non-demented elderly in acute human post-mortem cortical tissue
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.03.18.995332
DO 10.1101/2020.03.18.995332
A1 Alsema, Astrid M.
A1 Jiang, Qiong
A1 Kracht, Laura
A1 Gerrits, Emma
A1 Dubbelaar, Marissa L.
A1 Miedema, Anneke
A1 Brouwer, Nieske
A1 Woodbury, Maya
A1 Wachter, Astrid
A1 Xi, Hualin S.
A1 Möller, Thomas
A1 Biber, Knut P.
A1 Kooistra, Susanne M.
A1 Boddeke, Erik W.G.M
A1 Eggen, Bart J.L.
YR 2020
UL http://biorxiv.org/content/early/2020/03/19/2020.03.18.995332.abstract
AB Microglia are the tissue-resident macrophages of the central nervous system (CNS). Recent studies based on bulk and single-cell RNA sequencing in mice indicate high relevance of microglia with respect to risk genes and neuro-inflammation in Alzheimer’s disease. Here, we investigated microglia transcriptomes at bulk and single cell level in non-demented elderly and AD donors using acute human post-mortem cortical brain samples. We identified 9 human microglial subpopulations with heterogeneity in gene expression. Notably, gene expression profiles and subcluster composition of microglia did not differ between AD donors and non-demented elderly in bulk RNA sequencing nor in single-cell sequencing.