TY - JOUR T1 - PacBio SMRT-based full-length transcriptome data of <em>Ascosphaera apis</em> mycelium and spore JF - bioRxiv DO - 10.1101/2020.03.19.998625 SP - 2020.03.19.998625 AU - Yu Du AU - Jie Wang AU - Huazhi Chen AU - Xiaoxue Fan AU - Zhiwei Zhu AU - Yuanchan Fan AU - Haibin Jiang AU - Cuiling Xiong AU - Yanzhen Zheng AU - Dafu Chen AU - Rui Guo Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/03/20/2020.03.19.998625.abstract N2 - Ascosphaera apis is an entomopathogenic fungus that exclusively infects honeybee larvae, resulting in chalkbrood disease, a widespread fungal disease damaging the beekeeping industry all over the world. In this article, purified mycelia (Aam) and spores (Aas) of A. apis pure culture under lab condition were sequenced using PacBio Sequel platform. In total, 13,302,489 and 9,911,345 subreads were yielded from Aam and Aas, respectively; 394,142 and 274,928 circular consensus sequence (CCS) reads were identified as being full-length non-chimeric (FLNC) reads, with a mean length of 2820 bp and 2602 bp, respectively. Furthermore, 174,095 and 103,845 corrected isoforms were identified, with a N50 length of 3543 bp and 3262 bp, respectively. The reported full-length transcriptome data of A. apis mycelium and spore will provide a valuable resource for improvement of genome and transcriptome annotations as well as better understanding of transcript structure such as alternative splicing and polyadenylation.Value of the dataCurrent dataset offers a set of high-quality full-length transcripts of A. apis.The data can facilitate the improvement of A. apis genome and transcriptome annotations.This dataset benefits further exploration of alternative splicing and polyadenylation of A. apis mRNAs. ER -