RT Journal Article
SR Electronic
T1 CRISPR/Cas9-based genome editing in the silverleaf whitefly (<em>Bemisia tabaci</em>)
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.03.18.996801
DO 10.1101/2020.03.18.996801
A1 Chan C. Heu
A1 Francine M. McCullough
A1 Junbo Luan
A1 Jason L. Rasgon
YR 2020
UL http://biorxiv.org/content/early/2020/03/20/2020.03.18.996801.abstract
AB Bemisia tabaci cryptic species Middle East-Asia Minor I (MEAM1) is a serious agricultural polyphagous insect pest, and vector of numerous plant viruses, causing major worldwide economic losses. B. tabaci control is limited by lack of robust gene editing tools. Gene editing is difficult in B. tabaci due to small embryos that are technically challenging to inject, and which have high mortality post-injection. We developed a CRISPR/Cas9 gene editing protocol based on injection of vitellogenic adult females rather than embryos (“ReMOT Control”). We identified an ovary-targeting peptide ligand (“BtKV”) that, when fused to Cas9 and injected into adult females, transduced the ribonucleoprotein complex to the germline, resulting in efficient, heritable editing of the offspring genome. In contrast to embryo injection, adult injection is easy and does not require specialized equipment. Development of easy-to-use gene editing protocols for B. tabaci will allow researchers to apply the power of reverse genetic approaches to this species and will lead to novel control methods for this devastating pest insect.