PT  - JOURNAL ARTICLE
AU  - T. Ono
AU  - K. Kamada
AU  - R. Hayashi
AU  - A. R. Piacenti
AU  - C. Gabbutt
AU  - N. Sriwilaijaroen
AU  - H. Hiramatsu
AU  - Y. Kanai
AU  - K. Inoue
AU  - S. Nakakita
AU  - T. Kawahara
AU  - Y. Ie
AU  - Y. Watanabe
AU  - Y. Suzuki
AU  - S. Contera
AU  - K. Matsumoto
TI  - Lab-on-a-graphene-FET detection of key molecular events underpinning influenza virus infection and effect of antiviral drugs
AID  - 10.1101/2020.03.18.996884
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.03.18.996884
4099  - http://biorxiv.org/content/early/2020/03/20/2020.03.18.996884.short
4100  - http://biorxiv.org/content/early/2020/03/20/2020.03.18.996884.full
AB  - Small solid-state devices are candidates for accelerating biomedical assays/drug discovery, however their potential remains unfulfilled. Here, we demonstrate that graphene-field effect transistors (FET) can be used to successfully detect the key molecular events underlying viral infections and the effect of antiviral drugs. Our device success is achieved by bio-mimicking the host-cell surface during an influenza infection at the graphene channel. In-situ AFM confirms the biological interactions at the sialic acid-functionalized graphene: viral hemagglutinin (HA) binds to sialic acid, and neuraminidase (NA) reacts with the sialic acid-HA complex. The graphene-FET detects HA binding to sialic acid, and NA cleavage of sialic acid. The inhibitory effect of the drug “zanamivir” on NA-sialic acid interactions is monitored in real-time; the reaction rate constant of NA-sialic acid reaction was successfully determined. We demonstrate that graphene-FETs are powerful platforms for measurement of biomolecular interactions and contribute to future deployment of solid-state devices in drug discovery/biosensing.