RT Journal Article
SR Electronic
T1 Lab-on-a-graphene-FET detection of key molecular events underpinning influenza virus infection and effect of antiviral drugs
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.03.18.996884
DO 10.1101/2020.03.18.996884
A1 Ono, T.
A1 Kamada, K.
A1 Hayashi, R.
A1 Piacenti, A. R.
A1 Gabbutt, C.
A1 Sriwilaijaroen, N.
A1 Hiramatsu, H.
A1 Kanai, Y.
A1 Inoue, K.
A1 Nakakita, S.
A1 Kawahara, T.
A1 Ie, Y.
A1 Watanabe, Y.
A1 Suzuki, Y.
A1 Contera, S.
A1 Matsumoto, K.
YR 2020
UL http://biorxiv.org/content/early/2020/03/20/2020.03.18.996884.abstract
AB Small solid-state devices are candidates for accelerating biomedical assays/drug discovery, however their potential remains unfulfilled. Here, we demonstrate that graphene-field effect transistors (FET) can be used to successfully detect the key molecular events underlying viral infections and the effect of antiviral drugs. Our device success is achieved by bio-mimicking the host-cell surface during an influenza infection at the graphene channel. In-situ AFM confirms the biological interactions at the sialic acid-functionalized graphene: viral hemagglutinin (HA) binds to sialic acid, and neuraminidase (NA) reacts with the sialic acid-HA complex. The graphene-FET detects HA binding to sialic acid, and NA cleavage of sialic acid. The inhibitory effect of the drug “zanamivir” on NA-sialic acid interactions is monitored in real-time; the reaction rate constant of NA-sialic acid reaction was successfully determined. We demonstrate that graphene-FETs are powerful platforms for measurement of biomolecular interactions and contribute to future deployment of solid-state devices in drug discovery/biosensing.