PT - JOURNAL ARTICLE AU - Hong Tan AU - Ruth E. Fulton AU - Wen-Hai Chou AU - Denise A. Birkholz AU - Meridee P. Mannino AU - David M. Yamaguchi AU - Steven G. Britt TI - <em>Drosophila</em> R8 photoreceptor cell subtype specification requires <em>Notch</em> and <em>hibris</em> AID - 10.1101/398222 DP - 2018 Jan 01 TA - bioRxiv PG - 398222 4099 - http://biorxiv.org/content/early/2018/08/22/398222.short 4100 - http://biorxiv.org/content/early/2018/08/22/398222.full AB - Cell differentiation and cell fate determination in sensory systems are essential for stimulus discrimination and coding of environmental stimuli. Color vision is based on the differential color sensitivity of retinal photoreceptors, however the developmental programs that control photoreceptor cell differentiation and specify color sensitivity are poorly understood. In Drosophila melanogaster, there is evidence that the color sensitivity of different photoreceptors in the compound eye is regulated by inductive signals between cells, but the exact nature of these signals and how they are propagated remains unknown. We conducted a genetic screen to identify additional regulators of this process and identified a novel mutation in the hibris gene. hibris encodes an irre cell recognition module protein (IRM). These immunoglobulin super family cell adhesion molecules include human neph and nephrin (NPHS1). hibris is expressed dynamically in the developing Drosophila melanogaster eye and loss-of-function mutations give rise to a diverse range of mutant phenotypes including disruption of the specification of R8 photoreceptors cell diversity. The specification of blue or green sensitivity in R8 cells is also dependent upon Notch signaling. We demonstrate that hibris is required within the retina, non-cell autonomously for these effects, suggesting an additional layer of complexity in the signaling process that produces paired expression of opsin genes in adjacent R7 and R8 photoreceptor cells.Author Summary As humans, our ability to distinguish different colors is dependent upon the presence of three different types of cone cell neurons in the retina of the eye. The cone cells express blue, green or red absorbing visual pigments that detect and discriminate between these colors. The principle of color discrimination by neurons “tuned” to different colors is an evolutionarily conserved specialization that occurs in many different animals. This specialization requires 1) visual pigments that detect different colors and 2) a developmental program that regulates the expression of these pigments in different types of cells. In this study we discovered that the fruit fly (Drosophila melanogaster) gene hibris is required for the developmental program that produces blue sensitive neurons in the fly retina. When we over-expressed hibris throughout the developing retina, extra blue sensitive cells were produced. These results demonstrate that if there is not enough hibris, too few blue sensitive cells form, but if there is too much hibris, too many blue sensitive cells form. Finally, we discovered that the hibris gene does not act in color sensitive neurons of the retina themselves. This surprising discovery suggests that hibris may influence development of the retina in a completely new and different way.