RT Journal Article SR Electronic T1 Cold trapped - Correcting locomotion dependent observation biases in thermal preference of Drosophila JF bioRxiv FD Cold Spring Harbor Laboratory SP 400069 DO 10.1101/400069 A1 Diego Giraldo A1 Andrea K. Adden A1 Ilyas Kuhlemann A1 Heribert Gras A1 Bart R. H. Geurten YR 2018 UL http://biorxiv.org/content/early/2018/08/24/400069.abstract AB Sensing environmental temperatures is essential for the survival of ectothermic organisms. In Drosophila, two methodologies are used to study temperature preferences (TP) and the genes involved in thermosensation: two-choice assays and temperature gradients. Whereas two-choice assays reveal a relative TP, temperature gradients can identify the absolute Tp. One drawback of gradients is that small ectothermic animals are susceptible to cold-trapping: a physiological inability to move at the cold area of the gradient. Often cold-trapping cannot be avoided, biasing the resulting TP to lower temperatures. Two mathematical models were previously developed to correct for cold-trapping. These models, however, focus on group behaviour which can lead to overestimation of cold-trapping due to group aggregation. Here we present a mathematical model that estimates the behaviour of individual Drosophilain temperature gradients. The model takes the spatial dimension and temperature difference of the gradient into account, as well as the rearing temperature of the flies. Furthermore, it allows quantifying cold-trapping, reveals true TP, and differentiates between temperature preference and tolerance. Online simulation is hosted at http://igloo.uni-goettingen.de. The code can be accessed at https://github.com/zerotonin/igloo.AbbreviationsIGLOOIGLOO is a Gradient LOcomotion mOdelwt18CantonS adult flies reared at 18°C temperaturewt25CantonS adult flies reared at 25°C temperaturewt30CantonS adult flies reared at 30°C temperaturewtLCantonS larval flies reared at 25°C temperatureTPtemperature preferenceTRrearing temperatureTAambient temperatureTBbody temperatureCIconfidence interval