PT  - JOURNAL ARTICLE
AU  - Gun-Soo Park
AU  - Keunbon Ku
AU  - Seung-Hwa Baek
AU  - Seong Jun Kim
AU  - Seung Il Kim
AU  - Bum-Tae Kim
AU  - Jin-Soo Maeng
TI  - Development of Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) Assays Targeting SARS-CoV-2
AID  - 10.1101/2020.03.09.983064
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.03.09.983064
4099  - http://biorxiv.org/content/early/2020/03/24/2020.03.09.983064.short
4100  - http://biorxiv.org/content/early/2020/03/24/2020.03.09.983064.full
AB  - Epidemics of Coronavirus Disease 2019 (COVID-19) now have more than 100,000 confirmed cases worldwide. Diagnosis of COVID-19 is currently performed by RT-qPCR methods, but the capacity of RT-qPCR methods is limited by its requirement of high-level facilities and instruments. Here, we developed and evaluated RT-LAMP assays to detect genomic RNA of SARS-CoV-2, the causative virus of COVID-19. RT-LAMP assays in this study can detect as low as 100 copies of SARS-CoV-2 RNA. Cross-reactivity of RT-LAMP assays to other human Coronaviruses was not observed. We also adapted a colorimetric detection method for our RT-LAMP assay so that the tests potentially performed in higher throughput.