RT Journal Article
SR Electronic
T1 Genetically Encoded, Multivalent Liquid Glycan Array (LiGA)
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.03.24.997536
DO 10.1101/2020.03.24.997536
A1 Mirat Sojitra
A1 Susmita Sarkar
A1 Jasmine Maghera
A1 Emily Rodrigues
A1 Eric Carpenter
A1 Shaurya Seth
A1 Daniel Ferrer Vinals
A1 Nicholas Bennett
A1 Revathi Reddy
A1 Amira Khalil
A1 Xiaochao Xue
A1 Michael Bell
A1 Ruixiang Blake Zheng
A1 Chang-Chun Ling
A1 Todd L. Lowary
A1 James C. Paulson
A1 Matthew S. Macauley
A1 Ratmir Derda
YR 2020
UL http://biorxiv.org/content/early/2020/03/25/2020.03.24.997536.abstract
AB The Central Dogma of Biology does not allow for the study of glycans using DNA sequencing. We report a “Liquid Glycan Array” (LiGA) platform comprising a library of DNA ‘barcoded’ M13 virions that display 30-1500 copies of glycans per phage. A LiGA is synthesized by acylation of phage pVIII protein with a dibenzocyclooctyne, followed by ligation of azido-modified glycans. Pulldown of the LiGA with lectins followed by deep sequencing of the barcodes in the bound phage decodes the optimal structure and density of the recognized glycans. The LiGA is target agnostic and can measure the glycan-binding profile of lectins such as CD22 on cells in vitro and immune cells in a live mouse. From a mixture of multivalent glycan probes, LiGAs identifies the glycoconjugates with optimal avidity necessary for binding to lectins on living cells in vitro and in vivo; measurements that cannot be performed with canonical glass slide-based glycan arrays.