PT  - JOURNAL ARTICLE
AU  - Yu Du
AU  - Haibin Jiang
AU  - Huazhi Chen
AU  - Jie Wang
AU  - Yuanchan Fan
AU  - Xiaoxue Fan
AU  - Cuiling Xiong
AU  - Yanzhen Zheng
AU  - Dafu Chen
AU  - Rui Guo
TI  - Whole genome bisulfite sequencing dataset of mycelium and spore of chalkbrood disease pathogen <em>Ascosphaera apis</em>
AID  - 10.1101/2020.03.23.002980
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.03.23.002980
4099  - http://biorxiv.org/content/early/2020/03/25/2020.03.23.002980.short
4100  - http://biorxiv.org/content/early/2020/03/25/2020.03.23.002980.full
AB  - Chalkbrood, a widespread fungal disease of bee larvae, is caused by the fungus Ascosphaera apis. In this article, mecylia and spores of A. apis were respectively collected followed by DNA isolation, bisulfite conversion, cDNA library construction and next-generation sequencing. Using whole genome bisulfite sequencing (WGBS), 69,844,360 and 60,570,990 raw reads were yielded from Aam and Aas, and after quality control, 9,982,386,951 and 8,825,601,434 clean reads were obtained, respectively. In addition, 67,685,866 and 58,886,072 clean reads were mapped to the reference genome of A. apis, including 37,643,592 and 31,568,442 unique mapped clean reads, and 49,686 and 13,348 multiple mapped clean reads. Furthermore, after bisulfite treatment, the conversion ratio of clean reads from Aam and Aas were 99.38% and 99.51%, respectively. The WGBS data ducumented here contributes to genome-wide identification of 5mC methylation sites in A. apis and comparison of methylomes between mycelium and spore.Value of the dataThis dataset can be used for genome-wide identification of 5mC methylation sites in A. apis.The accessible data could be used to systematically compare methylomes between mycelium and spore of A. apis.Current data provides a useful resource for further study on DNA methylation-mediated mechanism underlying mycelium growth, spore germination and sexual reproduction of mycelium with the opposite sex.