RT Journal Article
SR Electronic
T1 Three-Dimensional Histology of Whole Zebrafish by Sub-Micron Synchrotron X-ray Micro-Tomography
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 392381
DO 10.1101/392381
A1 Yifu Ding
A1 Daniel J. Vanselow
A1 Maksim A. Yakovlev
A1 Spencer R. Katz
A1 Alex Y. Lin
A1 Darin P. Clark
A1 Phillip Vargas
A1 Xuying Xin
A1 Jean E. Copper
A1 Victor A. Canfield
A1 Khai C. Ang
A1 Yuxin Wang
A1 Xianghui Xiao
A1 Francesco De Carlo
A1 Damian B. van Rossum
A1 Patrick La Rivière
A1 Keith C. Cheng
YR 2018
UL http://biorxiv.org/content/early/2018/08/25/392381.abstract
AB Histological studies providing cellular insights into tissue architecture have been central to biological discovery and remain clinically invaluable today. Extending histology to three dimensions would be transformational for research and diagnostics. However, three-dimensional histology is impractical using current techniques. We have customized sample preparation, synchrotron X-ray tomographic parameters, and three-dimensional image analysis to allow for complete histological phenotyping using whole larval and juvenile zebrafish. The resulting digital zebrafish can be virtually sectioned and visualized in any plane. Whole-animal reconstructions at subcellular resolution also enable computational characterization of the zebrafish nervous system by region-specific detection of cell nuclei and quantitative assessment of individual phenotypic variation. Three-dimensional histological phenotyping has potential use in genetic and chemical screens, and in clinical and toxicological tissue diagnostics.One Sentence Summary Synchrotron X-ray micro-tomography can be used to rapidly create 3-dimensional images of fixed and stained specimens without sectioning, enabling computational histological phenotyping at cellular resolution.Abbreviations3Dthree-dimensionalH&Ehematoxylin & eosin2Dtwo-dimensionalmicro-CTX-ray micro-tomographydpfdays post-fertilizationPTAphosphotungstic acidRBCred blood cell