TY - JOUR T1 - Physical confinement impacts cellular phenotype within living materials JF - bioRxiv DO - 10.1101/2020.03.25.004887 SP - 2020.03.25.004887 AU - Hans Priks AU - Tobias Butelmann AU - Aleksandr Illarionov AU - Trevor G. Johnston AU - Christopher Fellin AU - Tarmo Tamm AU - Alshakim Nelson AU - Rahul Kumar AU - Petri-Jaan Lahtvee Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/03/25/2020.03.25.004887.abstract N2 - Additive manufacturing allows three-dimensional printing of polymeric materials together with cells, creating living materials for applications in biomedical research and biotechnology. However, understanding the cellular phenotype within living materials is lacking and a key limitation for their wider application. Herein, we present an approach to characterize the cellular phenotype within living materials. We immobilized the budding yeast Saccharomyces cerevisiae in three different photocross-linkable triblock polymeric hydrogels containing F127-bis-urethane methacrylate, F127-dimethacrylate, or poly(alkyl glycidyl ether)-dimethacrylate. Using optical and scanning electron microscopy, we showed that hydrogels based on these polymers were stable under physiological conditions, but yeast colonies showed differences in the interaction within the living materials. We found that the physical confinement, imparted by compositional and structural properties of the hydrogels, impacted the cellular phenotype by reducing the size of cells in living materials compared with suspension cells. These properties also contributed to the differences in immobilization patterns, growth of colonies, and colony coatings. We observed that a composition-dependent degradation of polymers was likely possible by cells residing in the living materials. In conclusion, our investigation highlights the need for a holistic understanding of the cellular response within hydrogels to facilitate the synthesis of application-specific polymers and the design of advanced living materials in the future. ER -