TY - JOUR T1 - A full cycle anti-viral drug screen identifies a clinical compound against adenovirus infection JF - bioRxiv DO - 10.1101/2020.03.24.006346 SP - 2020.03.24.006346 AU - Fanny Georgi AU - Fabien Kuttler AU - Luca Murer AU - Vardan Andriasyan AU - Robert Witte AU - Artur Yakimovich AU - Gerardo Turcatti AU - Urs F Greber Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/03/25/2020.03.24.006346.1.abstract N2 - Human adenoviruses (HAdVs) are fatal to immune-suppressed people, but no effective anti-HAdV therapy is available. Here, we present a novel image-based high-throughput screening (HTS) platform, which scores the full viral replication cycle from virus entry to dissemination of progeny. We analysed 1,280 small molecular compounds of the Prestwick Chemical Library (PCL) for interference with HAdV-C2 infection in a quadruplicate blinded format, followed by robust image analyses, and hit identification. We present the entire set of image-based screening data including all the images, and the image analysis and data processing pipelines, as deposited at the Image Data repository (IDR) 1, accession number idr0081. We identified Nelfinavir mesylate as an inhibitor of HAdV plaque formation, in agreement with the previous notion that Nelfinavir is ineffective in single round HAdV infection assays. Nelfinavir has been FDA-approved for anti-retroviral therapy in humans. Our results underscore the power of image-based multi-round infection assays in identifying viral inhibitors with clinical potential.BSAbovine serum albumin;BSFBiomolecular Screening Facility;CMVCytomegalovirus;DFT3′-Deoxy-3′-fluorothymidine;DMEMDulbecco Modified Eagle medium;DMSODimethyl sulfoxide;dpidays post infection;EPFLEcole Polytechnique Fédérale de Lausanne;FBSfetal bovine serum;GFPgreen fluorescent protein;HAdVHuman adenovirus;hpihours post infection;HTShigh-throughput screening;IDRThe Image Data Resource;LIMSLaboratory Information Management System;LUTLook up table;PCLPrestwick Chemical Library;PFApara-formaldehyde;pfuplaque forming units;RTroom temperature;SEstandard error;SDstandard deviation;UZHUniversity of Zurich ER -