PT  - JOURNAL ARTICLE
AU  - Richa Mishra
AU  - K Pandikannan
AU  - S Gangamma
AU  - Ashwin Ashok Raut
AU  - Himanshu Kumar
TI  - Imperative role of particulate matter in innate immunity during RNA virus infection
AID  - 10.1101/2020.03.28.013169
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.03.28.013169
4099  - http://biorxiv.org/content/early/2020/03/29/2020.03.28.013169.short
4100  - http://biorxiv.org/content/early/2020/03/29/2020.03.28.013169.full
AB  - Sensing of pathogens by specialized receptors is the hallmark of the innate immune response. Innate immune response also mounts a defense response against various allergens and pollutants including particulate matter present in the atmosphere. Air pollution has been included as the top threat to global health declared by WHO which aims to cover more than three billion people against health emergencies from 2019-2023. Particulate matter (PM), one of the major components of air pollution, is a significant risk factor for many human diseases and its adverse effects include morbidity and premature deaths throughout the world. Several clinical and epidemiological studies have identified a key link between the PM composition and the prevalence of respiratory and inflammatory disorders. However, the underlying molecular mechanism is not well understood. Here, we investigated the influence of air pollutant, PM10 during RNA virus infections using highly pathogenic avian influenza (HPAI). We thus characterized the transcriptomic profile of lung epithelial cell line, A549 treated with PM10 prior to infection with (HPAI) H5N1 influenza virus, which is known to severely affect the lung and cause respiratory damage. We found that PM10 regulates virus infectivity and enhances overall pathogenic burden in the lung cells. Additionally, the transcriptomic profile highlights the connection of host factors related to various metabolic pathways and immune responses which were dysregulated during virus infection. Overall our findings suggest a strong link between the prevalence of respiratory illness and the air quality.