RT Journal Article
SR Electronic
T1 High-throughput single-cell transcriptome profiling of plant cell types
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 402966
DO 10.1101/402966
A1 Christine N. Shulse
A1 Benjamin J. Cole
A1 Gina M. Turco
A1 Yiwen Zhu
A1 Siobhan M. Brady
A1 Diane E. Dickel
YR 2018
UL http://biorxiv.org/content/early/2018/08/29/402966.abstract
AB Single-cell transcriptome analysis of heterogeneous tissues can provide high-resolution windows into the genomic basis and spatiotemporal dynamics of developmental processes. Here we demonstrate the feasibility of high-throughput single-cell RNA sequencing of plant tissue using the Drop-seq approach. Profiling of >4,000 individual cells from the Arabidopsis root provides transcriptomes and marker genes for a diversity of cell types and illuminates the gene expression changes that occur across endodermis development.