RT Journal Article
SR Electronic
T1 Chandelier cell anatomy and function reveal a variably distributed but common signal
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.03.31.018952
DO 10.1101/2020.03.31.018952
A1 Casey M. Schneider-Mizell
A1 Agnes L. Bodor
A1 Forrest Collman
A1 Derrick Brittain
A1 Adam A. Bleckert
A1 Sven Dorkenwald
A1 Nicholas L. Turner
A1 Thomas Macrina
A1 Kisuk Lee
A1 Ran Lu
A1 Jingpeng Wu
A1 Jun Zhuang
A1 Anirban Nandi
A1 Brian Hu
A1 JoAnn Buchanan
A1 Marc M. Takeno
A1 Russel Torres
A1 Gayathri Mahalingam
A1 Daniel J. Bumbarger
A1 Yang Li
A1 Tom Chartrand
A1 Nico Kemnitz
A1 William M. Silversmith
A1 Dodam Ih
A1 Jonathan Zung
A1 Aleksandar Zlateski
A1 Ignacio Tartavull
A1 Sergiy Popovych
A1 William Wong
A1 Manuel Castro
A1 Chris S. Jordan
A1 Emmanouil Froudarakis
A1 Lynne Becker
A1 Shelby Suckow
A1 Jacob Reimer
A1 Andreas S. Tolias
A1 Costas Anastassiou
A1 H. Sebastian Seung
A1 R. Clay Reid
A1 Nuno Maçarico da Costa
YR 2020
UL http://biorxiv.org/content/early/2020/04/01/2020.03.31.018952.abstract
AB The activity and connectivity of inhibitory cells has a profound impact on the operation of neuronal networks. While the average connectivity of many inhibitory cell types has been characterized, we still lack an understanding of how individual interneurons distribute their synapses onto their targets and how heterogeneous the inhibition is onto different individual excitatory neurons. Here, we use large-scale volumetric electron microscopy (EM) and functional imaging to address this question for chandelier cells in layer 2/3 of mouse visual cortex. Using dense morphological reconstructions from EM, we mapped the complete chandelier input onto 153 pyramidal neurons. We find that the number of input synapses is highly variable across the population, but the variability is correlated with structural features of the target neuron: soma depth, soma size, and the number of perisomatic synapses received. Functionally, we found that chandelier cell activity in vivo was highly correlated and tracks pupil diameter, a proxy for arousal state. We propose that chandelier cells provide a global signal whose strength is individually adjusted for each target neuron. This approach, combining comprehensive structural analysis with functional recordings of identified cell types, will be a powerful tool to uncover the wiring rules across the diversity of cortical cell types.