RT Journal Article
SR Electronic
T1 Selective Cell Propagation via Micropatterning of Thermal-activated Hydrogel
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.04.01.019208
DO 10.1101/2020.04.01.019208
A1 Jeffrey C.Y. Chiu
A1 Joyce A. Teodoro
A1 Jeong Hyun Lee
A1 Kerryn Matthews
A1 Simon P. Duffy
A1 Hongshen Ma
YR 2020
UL http://biorxiv.org/content/early/2020/04/01/2020.04.01.019208.abstract
AB The ability to selectively propagate specific cells is fundamentally important to the development of clonal cell populations. Current methods rely on techniques such as limiting dilution, colony picking, and flow cytometry to transfer single cells into single wells, resulting in workflows that are low-throughput, slowed by propagation kinetics, and susceptible to contamination. Here, we developed a method, called selective laser gelation (SLG), to micropattern hydrogels in cell culture media in order to encapsulate specific cells to selectively arrest their growth. This process relies on the inverse gelation of methylcellulose, which forms a hydrogel when heated rather than cooled. Local heating using an infrared laser enables hydrogel micropatterning, while phase transition hysteresis retains the hydrogel after laser excitation. As a demonstration, we used this approach to selectively propagate transgenic CHO cells with increased antibody productivity. More generally, hydrogel micropatterning provides a simple and non-contact method to selective propagation of cells based on features identified by imaging.One Sentence Summary Inverse gelation of methylcellulose enables hydrogel micropatterning to selectively propagate cells identified by microscopy.