RT Journal Article
SR Electronic
T1 Human cortical expansion involves diversification and specialization of supragranular intratelencephalic-projecting neurons
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.03.31.018820
DO 10.1101/2020.03.31.018820
A1 Jim Berg
A1 Staci A. Sorensen
A1 Jonathan T. Ting
A1 Jeremy A. Miller
A1 Thomas Chartrand
A1 Anatoly Buchin
A1 Trygve E. Bakken
A1 Agata Budzillo
A1 Nick Dee
A1 Song-Lin Ding
A1 Nathan W. Gouwens
A1 Rebecca D. Hodge
A1 Brian Kalmbach
A1 Changkyu Lee
A1 Brian R. Lee
A1 Lauren Alfiler
A1 Katherine Baker
A1 Eliza Barkan
A1 Allison Beller
A1 Kyla Berry
A1 Darren Bertagnolli
A1 Kris Bickley
A1 Jasmine Bomben
A1 Thomas Braun
A1 Krissy Brouner
A1 Tamara Casper
A1 Peter Chong
A1 Kirsten Crichton
A1 Rachel Dalley
A1 Rebecca de Frates
A1 Tsega Desta
A1 Samuel Dingman Lee
A1 Florence D’Orazi
A1 Nadezhda Dotson
A1 Tom Egdorf
A1 Rachel Enstrom
A1 Colin Farrell
A1 David Feng
A1 Olivia Fong
A1 Szabina Furdan
A1 Anna A. Galakhova
A1 Clare Gamlin
A1 Amanda Gary
A1 Alexandra Glandon
A1 Jeff Goldy
A1 Melissa Gorham
A1 Natalia A. Goriounova
A1 Sergey Gratiy
A1 Lucas Graybuck
A1 Hong Gu
A1 Kristen Hadley
A1 Nathan Hansen
A1 Tim S. Heistek
A1 Alex M. Henry
A1 Djai B. Heyer
A1 DiJon Hill
A1 Chris Hill
A1 Madie Hupp
A1 Tim Jarsky
A1 Sara Kebede
A1 Lisa Keene
A1 Lisa Kim
A1 Mean-Hwan Kim
A1 Matthew Kroll
A1 Caitlin Latimer
A1 Boaz P. Levi
A1 Katherine E. Link
A1 Matthew Mallory
A1 Rusty Mann
A1 Desiree Marshall
A1 Michelle Maxwell
A1 Medea McGraw
A1 Delissa McMillen
A1 Erica Melief
A1 Eline J. Mertens
A1 Leona Mezei
A1 Norbert Mihut
A1 Stephanie Mok
A1 Gabor Molnar
A1 Alice Mukora
A1 Lindsay Ng
A1 Kiet Ngo
A1 Philip R. Nicovich
A1 Julie Nyhus
A1 Gaspar Olah
A1 Aaron Oldre
A1 Victoria Omstead
A1 Attila Ozsvar
A1 Daniel Park
A1 Hanchuan Peng
A1 Trangthanh Pham
A1 Christina A. Pom
A1 Lydia Potekhina
A1 Ramkumar Rajanbabu
A1 Shea Ransford
A1 David Reid
A1 Christine Rimorin
A1 Augustin Ruiz
A1 David Sandman
A1 Josef Sulc
A1 Susan M. Sunkin
A1 Aaron Szafer
A1 Viktor Szemenyei
A1 Elliot R. Thomsen
A1 Michael Tieu
A1 Amy Torkelson
A1 Jessica Trinh
A1 Herman Tung
A1 Wayne Wakeman
A1 Katelyn Ward
A1 René Wilbers
A1 Grace Williams
A1 Zizhen Yao
A1 Jae-Geun Yoon
A1 Costas Anastassiou
A1 Anton Arkhipov
A1 Pal Barzo
A1 Amy Bernard
A1 Charles Cobbs
A1 Philip C. de Witt Hamer
A1 Richard G. Ellenbogen
A1 Luke Esposito
A1 Manuel Ferreira
A1 Ryder P. Gwinn
A1 Michael J. Hawrylycz
A1 Patrick R. Hof
A1 Sander Idema
A1 Allan R. Jones
A1 C.Dirk Keene
A1 Andrew L. Ko
A1 Gabe J. Murphy
A1 Lydia Ng
A1 Jeffrey G. Ojemann
A1 Anoop P. Patel
A1 John W. Phillips
A1 Daniel L. Silbergeld
A1 Kimberly Smith
A1 Bosiljka Tasic
A1 Rafael Yuste
A1 Idan Segev
A1 Christiaan P.J. de Kock
A1 Huibert D. Mansvelder
A1 Gabor Tamas
A1 Hongkui Zeng
A1 Christof Koch
A1 Ed S. Lein
YR 2020
UL http://biorxiv.org/content/early/2020/04/02/2020.03.31.018820.abstract
AB The neocortex is disproportionately expanded in human compared to mouse, both in its total volume relative to subcortical structures and in the proportion occupied by supragranular layers that selectively make connections within the cortex and other telencephalic structures. Single-cell transcriptomic analyses of human and mouse cortex show an increased diversity of glutamatergic neuron types in supragranular cortex in human and pronounced gradients as a function of cortical depth. To probe the functional and anatomical correlates of this transcriptomic diversity, we describe a robust Patch-seq platform using neurosurgically-resected human tissues. We characterize the morphological and physiological properties of five transcriptomically defined human glutamatergic supragranular neuron types. Three of these types have properties that are specialized compared to the more homogeneous properties of transcriptomically defined homologous mouse neuron types. The two remaining supragranular neuron types, located exclusively in deep layer 3, do not have clear mouse homologues in supragranular cortex but are transcriptionally most similar to deep layer mouse intratelencephalic-projecting neuron types. Furthermore, we reveal the transcriptomic types in deep layer 3 that express high levels of non-phosphorylated heavy chain neurofilament protein that label long-range neurons known to be selectively depleted in Alzheimer’s disease. Together, these results demonstrate the power of transcriptomic cell type classification, provide a mechanistic underpinning for increased complexity of cortical function in human cortical evolution, and implicate discrete transcriptomic cell types as selectively vulnerable in disease.