TY - JOUR T1 - Deregulated mito-nuclear communication alters chromatin plasticity and differentiation potential of mesenchymal stem cells upon ageing JF - bioRxiv DO - 10.1101/2020.04.02.022293 SP - 2020.04.02.022293 AU - Andromachi Pouikli AU - Swati Parekh AU - Monika Maleszewska AU - Maarouf Baghdadi AU - Ignacio Tripodi AU - Chrysa Nikopoulou AU - Kat Folz-Donahue AU - Yvonne Hinze AU - Andrea Mesaros AU - Patrick Giavalisco AU - Robin Dowell AU - Linda Partridge AU - Peter Tessarz Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/04/03/2020.04.02.022293.abstract N2 - Ageing is accompanied by a general decline in the function of many cellular pathways, with metabolic alterations, epigenetic modifications, and stem cell exhaustion representing three important hallmarks of the ageing process. However, whether these pathways are causally or functionally related at a molecular level remains poorly understood. Here, we use bone marrow-derived mesenchymal stem cells (MSCs) isolated from young and old mice to address how age-dependent changes in metabolism and epigenetics are linked and how they impact on the ageing transcriptome and differentiation potential. Given that MSCs maintain specific age-associated properties even under prolonged culture conditions, such as the age-dependent decrease in osteogenic differentiation, they are an excellent model to investigate in vitro the connection of ageing hallmarks on a mechanistic level. In this study, we demonstrate that upon ageing, osteogenic potential of MSCs declines as a consequence of deregulated mito-nuclear communication, mediated by decreased levels of the citrate carrier (CiC). Age-dependent down-regulation of CiC results in acetyl-CoA trapping within mitochondria, hypo-acetylation of histones and chromatin compaction. Together, these changes lead to an altered transcriptional output and are responsible for the reduced differentiation capacity into osteoblasts. Strikingly, short-term supplementation of aged cells with acetate, an exogenous source for cytosolic acetyl-CoA production, rescues not only the age-associated reduction of histone acetylation, but also the osteogenesis defect, representing a potential target for in vitro MSC rejuvenation. ER -