TY - JOUR T1 - Genetic interaction between profilin and myosin II reveals a potential role for myosin II in actin filament disassembly <em>in vivo</em> JF - bioRxiv DO - 10.1101/2020.04.03.023705 SP - 2020.04.03.023705 AU - Paola Zambon AU - Saravanan Palani AU - Shekhar Sanjay Jadhav AU - Pananghat Gayathri AU - Mohan K. Balasubramanian Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/04/04/2020.04.03.023705.abstract N2 - The actin cytoskeleton plays a variety of roles in eukaryotic cell physiology, ranging from cell polarity and migration to cytokinesis. Key to the function of the actin cytoskeleton is the mechanisms that control its assembly, stability, and turnover. Through genetic analyses in fission yeast, we found that, myo2-S1 (myo2-G515D), a myosin II mutant allele was capable of rescuing lethality caused by compromise of mechanisms involved in actin cable / ring assembly and stability. The mutation in myo2-S1 affects the activation loop of Myosin II, which is involved in physical interaction with subdomain 1 of actin and in stimulating the ATPase activity of Myosin. Consistently, actomyosin rings in myo2-S1 cell ghosts were severely compromised in contraction upon ATP addition, suggesting that Myo2-S1p was defective in actin binding and / or motor activity. These studies strongly suggest a role for Myo2p in actin cytoskeletal disassembly and turnover, and that compromise of this activity leads to genetic suppression of mutants defective in actin cable assembly / stability. ER -