TY - JOUR T1 - Microinjection into the <em>Caenorhabditis elegans</em> embryo using an uncoated glass needle enables cell lineage visualization and reveals cell-non-autonomous adhesion control JF - bioRxiv DO - 10.1101/406991 SP - 406991 AU - Yohei Kikuchi AU - Akatsuki Kimura Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/09/03/406991.abstract N2 - Microinjection is a useful method in cell biology, with which exogenous substances are introduced into a cell in a location- and time-specific manner. The Caenorhabditis elegans embryo is an important model system for cell and developmental biology. Applying microinjection to the C. elegans embryo had been difficult due to the rigid eggshell surrounding the embryo. In 2013, microinjection method using a carbon-coated quartz needle for the C. elegans embryo was reported. To prepare the needle, unfortunately, special equipment is required and thus a limited number of researchers can use this method. In this study, we established a method for the microinjection of drugs, dyes, and microbeads into the C. elegans embryo using an uncoated glass needle that can be produced in a general laboratory. This method enabled us to easily detect cell lineage up to adult stages by injecting a fluorescent dye into a blastomere. We also found a cell-non-autonomous control mechanism of cell adhesion; specifically, the injection of an actin inhibitor into one cell at the 2-cell stage enhanced adhesion between daughter cells of the other cell. Our microinjection method is expected to be used for broad studies and could facilitate various discoveries using C. elegans.AbbreviationEBegg bufferSEMscanning electron microscopySGMShelton’s growth medium ER -