RT Journal Article
SR Electronic
T1 Concerted localization-resets precede YAP-dependent transcription
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 539049
DO 10.1101/539049
A1 Rajarshi P. Ghosh
A1 J. Matthew Franklin
A1 Quanming Shi
A1 Michael P. Reddick
A1 Jan T. Liphardt
YR 2020
UL http://biorxiv.org/content/early/2020/04/05/539049.abstract
AB Yes-associated protein 1 (YAP) is a transcriptional regulator with critical roles in mechanotransduction, organ size control, and regeneration. Here, using new tools for real-time visualization of native YAP and target gene transcription dynamics, we show that a cycle of fast exodus of nuclear YAP to the cytoplasm followed by fast reentry to the nucleus (“localization-resets”) activates YAP target genes. These “resets” could be induced by calcium signaling, modulation of actomyosin contractility, or mitosis. Using nascent-transcription reporter knock-ins of YAP target genes, we observed a strict association between these resets and downstream transcription. Oncogenically-transformed transformed cell lines lacked localization-resets and instead showed dramatically elevated rates of nucleocytoplasmic shuttling of YAP, suggesting an escape from compartmentalization-based control. The single-cell localization and transcription traces suggest that YAP activity is not a simple linear function of nuclear enrichment and point to a new model of transcriptional activation based on nucleocytoplasmic exchange properties of YAP.