PT  - JOURNAL ARTICLE
AU  - Maria Montserrat Garcia Romero
AU  - Gareth McCathie
AU  - Philip Jankun
AU  - Henry Hamilton Roehl
TI  - Damage-induced reactive oxygen species enable zebrafish tail regeneration by repositioning of Hedgehog expressing cells
AID  - 10.1101/380378
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 380378
4099  - http://biorxiv.org/content/early/2018/09/03/380378.short
4100  - http://biorxiv.org/content/early/2018/09/03/380378.full
AB  - Aquatic vertebrates have a remarkable ability to regenerate limbs and tails after amputation. Previous studies indicate that reactive oxygen species (ROS) signaling initiates regeneration, but the mechanism by which this takes place is poorly understood. Developmental signalling pathways have been shown to have pro-regenerative roles in many systems. However, whether these are playing roles that are specific to regeneration, or are simply recapitulating their developmental functions is unclear. We have analysed zebrafish larval tail regeneration and find evidence that ROS released upon wounding cause repositioning of notochord cells to the damage site. These cells secrete Hedgehog ligands which are required for regeneration. Hedgehog signalling is not required for normal tail development suggesting that it has a regeneration specific role. Our results provide a model for how ROS initiate tail regeneration, and indicate that developmental signaling pathways can play regenerative functions that are not directly related to their developmental roles.