PT  - JOURNAL ARTICLE
AU  - Laura Mincarelli
AU  - Vladimir Uzun
AU  - Stuart A. Rushworth
AU  - Wilfried Haerty
AU  - Iain C. Macaulay
TI  - Combined single-cell gene and isoform expression analysis in haematopoietic stem and progenitor cells
AID  - 10.1101/2020.04.06.027474
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.04.06.027474
4099  - http://biorxiv.org/content/early/2020/04/06/2020.04.06.027474.short
4100  - http://biorxiv.org/content/early/2020/04/06/2020.04.06.027474.full
AB  - Single-cell RNA sequencing (scRNA-seq) enables gene expression profiling and characterization of novel cell types within heterogeneous cell populations. However, most approaches cannot detect alternatively spliced transcripts, which can profoundly shape cell phenotype by generating functionally distinct proteins from the same gene. Here, we integrate short- and long-read scRNA-seq of hematopoietic stem and progenitor cells to characterize changes in cell type abundance, gene and isoform expression during differentiation and ageing.