RT Journal Article
SR Electronic
T1 Combined single-cell gene and isoform expression analysis in haematopoietic stem and progenitor cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.04.06.027474
DO 10.1101/2020.04.06.027474
A1 Laura Mincarelli
A1 Vladimir Uzun
A1 Stuart A. Rushworth
A1 Wilfried Haerty
A1 Iain C. Macaulay
YR 2020
UL http://biorxiv.org/content/early/2020/04/06/2020.04.06.027474.abstract
AB Single-cell RNA sequencing (scRNA-seq) enables gene expression profiling and characterization of novel cell types within heterogeneous cell populations. However, most approaches cannot detect alternatively spliced transcripts, which can profoundly shape cell phenotype by generating functionally distinct proteins from the same gene. Here, we integrate short- and long-read scRNA-seq of hematopoietic stem and progenitor cells to characterize changes in cell type abundance, gene and isoform expression during differentiation and ageing.