RT Journal Article
SR Electronic
T1 Subthreshold Voltage Analysis Demonstrates Neuronal Cell-Surface Sialic Acids Modulate Excitability and Network Integration
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.04.07.030866
DO 10.1101/2020.04.07.030866
A1 Kulkarni, Rishikesh U.
A1 Wang, Catherine L.
A1 Bertozzi, Carolyn R.
YR 2020
UL http://biorxiv.org/content/early/2020/04/08/2020.04.07.030866.abstract
AB All neurons are covered in a thick layer of carbohydrates called glycans. Glycans are modified during neurological processes and are thought to play a role in neuronal communication. We develop a voltage imaging platform for analyzing functional connectivity changes using simultaneous voltage recordings in small populations of neurons. We validate this platform using a culture model of development as well as with several pharmacological interventions. Using this platform, we show that ablation of SNA-binding glycans results in loss of functional connectivity in mouse hippocampal neurons, while ablation of MAL II binding glycans minimally perturbs functional connectivity. Altogether, our data reveal that subpopulations of glycans play different roles in maintenance of electrophysiology and provide a platform for modeling changes in functional connectivity with simultaneous voltage recordings.