RT Journal Article
SR Electronic
T1 Budding yeast complete DNA replication after chromosome segregation begins
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 407957
DO 10.1101/407957
A1 Tsvetomira Ivanova
A1 Michael Maier
A1 Alsu Missarova
A1 Céline Ziegler-Birling
A1 Lucas B. Carey
A1 Manuel Mendoza
YR 2018
UL http://biorxiv.org/content/early/2018/09/05/407957.abstract
AB To faithfully transmit genetic information, cells must replicate their entire genome before division. This is thought to be ensured by the temporal separation of replication and chromosome segregation. Here we show that in a substantial fraction of unperturbed yeast cells, DNA replication finishes during anaphase, late in mitosis. High cyclin-Cdk activity inhibits replication in metaphase, and the decrease in cyclin-Cdk activity during mitotic exit allows DNA replication to finish at difficult-to-replicate regions. Replication during late mitosis correlates with elevated mutation rates, including copy number variation. Thus, yeast cells temporally overlap replication and chromosome segregation during normal growth, possibly allowing cells to maximize population-level growth rate while simultaneously exploring greater genetic space.One Sentence Summary Completion of DNA replication is coupled to downregulation of Cyclin-Dependent Kinase during mitotic exit.